7DIC

Mycoplasma genitalium RNase R in complex with single-stranded RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease R	polymer	747	85595.6	1	UniProt (P47350) Pfam (PF00773) Pfam (PF00575)	Mycoplasma genitalium G37	RNase R,VacB protein homolog
2	B (C)	RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*A)-3')	polymer	9	2917.9	1		Mycoplasma genitalium G37
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D, E (A, C)	water	water		18.0	128	Chemie (HOH)

Sequence modifications

A: 1 - 725 (UniProt: P47350)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
Ile -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Ala 284	Asp 284	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88513.4
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	88537.8

*Water molecules are not included.