7DIC

Mycoplasma genitalium RNase R in complex with single-stranded RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	68.258, 68.258, 354.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.266 - 2.242
R-factor	0.2174
Rwork	0.215
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xgu
RMSD bond length	0.012
RMSD bond angle	1.532
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2148)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.240	2.240
Rmerge	0.098	2.473
Number of reflections	41557	2028
<I/σ(I)>	18	1.3
Completeness [%]	99.4
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	100 mM KCl, 100 mM HEPES (pH 6.8), 15% PEG 5000MME