7DHR

Cryo-EM structure of the full agonist isoprenaline-bound beta2 adrenergic receptor-Gs protein complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45725.5	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (Bovine)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	78	8805.2	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
4	D (N)	Nb35 nanobody	polymer	157	17057.3	1		Lama glama
5	E (R)	Beta-2 adrenergic receptor	polymer	367	42042.9	1	UniProt (P07550) Pfam (PF00001)	Homo sapiens (Human)	Beta-2 adrenoreceptor,Beta-2 adrenoceptor
6	F (R)	ISOPRENALINE	non-polymer		211.3	1	Chemie (5FW)

Sequence modifications

G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 68	Cys 68	conflict

R: 1 - 348 (UniProt: P07550)

PDB	External Database	Details
Met -23	-	initiating methionine
Lys -22	-	expression tag
Thr -21	-	expression tag
Ile -20	-	expression tag
Ile -19	-	expression tag
Ala -18	-	expression tag
Leu -17	-	expression tag
Ser -16	-	expression tag
Tyr -15	-	expression tag
Ile -14	-	expression tag
Phe -13	-	expression tag
Ser -12	-	expression tag
Leu -11	-	expression tag
Val -10	-	expression tag
Phe -9	-	expression tag
Ala -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
Arg 16	Gly 16	engineered mutation
Gln 27	Glu 27	engineered mutation
Val 77	Cys 77	engineered mutation
Thr 96	Met 96	engineered mutation
Thr 98	Met 98	engineered mutation
Trp 122	Glu 122	engineered mutation
Val 125	Cys 125	engineered mutation
Glu 187	Asn 187	engineered mutation
-	Leu 245	deletion
-	Ser 246	deletion
-	Gln 247	deletion
-	Val 248	deletion
-	Glu 249	deletion
-	Gln 250	deletion
-	Asp 251	deletion
-	Gly 252	deletion
-	Arg 253	deletion
-	Thr 254	deletion
-	Gly 255	deletion
-	His 256	deletion
-	Gly 257	deletion
-	Leu 258	deletion
-	Arg 259	deletion
Ala 265	Cys 265	engineered mutation
Ala 285	Cys 285	engineered mutation
Ser 327	Cys 327	engineered mutation
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	151047.8
Non-Polymers*	Number of molecules	1
	Total formula weight	211.3
All*	Total formula weight	151259.0

*Water molecules are not included.