7DH5
Dog beta3 adrenergic receptor bound to mirabegron in complex with a miniGs heterotrimer
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 249 | 28964.7 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P54311) Pfam (PF00400) | Rattus norvegicus (Rat) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7547.7 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
| 4 | D (N) | nanobody Nb35 | polymer | 137 | 15015.7 | 1 | unidentified | ||
| 5 | E (R) | Beta-3 adrenergic receptor | polymer | 395 | 42441.3 | 1 | UniProt (O02662) Pfam (PF00001) | Canis lupus familiaris (Dog) | Beta-3 adrenoreceptor,Beta-3 adrenoceptor |
| 6 | F (R) | 2-(2-azanyl-1,3-thiazol-4-yl)-N-[4-[2-[[(2R)-2-oxidanyl-2-phenyl-ethyl]amino]ethyl]phenyl]ethanamide | non-polymer | 396.5 | 1 | Chemie (H6U) |
Sequence modifications
A: 5 - 195 (UniProt: P63092)
A: 204 - 384 (UniProt: P63092)
B: 2 - 340 (UniProt: P54311)
G: 1 - 67 (UniProt: P63212)
R: 2 - 379 (UniProt: O02662)
| PDB | External Database | Details |
|---|---|---|
| Asp 49 | Gly 49 | conflict |
| Asn 50 | Glu 50 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 196 | - | linker |
| Gly 197 | - | linker |
| Ser 198 | - | linker |
| Gly 199 | - | linker |
| Gly 200 | - | linker |
| Ser 201 | - | linker |
| Gly 202 | - | linker |
| Gly 203 | - | linker |
| Asp 249 | Ala 249 | conflict |
| Asp 252 | Ser 252 | conflict |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Ala 362 | Ile 372 | conflict |
| Ile 365 | Val 375 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 68 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Met 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Glu 380 | - | expression tag |
| Asn 381 | - | expression tag |
| Leu 382 | - | expression tag |
| Tyr 383 | - | expression tag |
| Phe 384 | - | expression tag |
| Gln 385 | - | expression tag |
| Gly 386 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 132713.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 396.5 | |
| All* | Total formula weight | 133110.3 |
