7DAD

EPD in complex with tubulin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (Q2XVP4) Pfam (PF00091) Pfam (PF03953)	Sus scrofa (Pig)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D (B, D)	Tubulin beta chain	polymer	445	49999.9	2	UniProt (A0A287AGU7) Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (P02554)	Sus scrofa (Pig)
3	E (E)	Stathmin-4	polymer	143	16844.2	1	UniProt (P63042) Pfam (PF00836)	Mus musculus (Mouse)	Stathmin-like protein B3,RB3
4	F (F)	Tubulin tyrosine ligase	polymer	384	44378.5	1	UniProt (E1BQ43)	Gallus gallus (Chicken)
5	G, R, U (A, C, D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	3	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
6	H, M, Q, S, X (A, B, C, F)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG) PubChem (888)
7	I, K, N, T (A, B, C)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA) PubChem (271)
8	J (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
9	L (B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
10	O, P (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES) PubChem (4478249)
11	V (D)	EPOTHILONE D	non-polymer		491.7	1	Chemie (EPD) PubChem (21733151) PubChem (23308419) PubChem (23644491) PubChem (29979352) PubChem (44184905) PubChem (45039144) PubChem (46781478) PubChem (46936246) PubChem (148190) PubChem (396644) PubChem (59044167) PubChem (59077231) PubChem (59104617) PubChem (59131082) PubChem (59903453) PubChem (68412194) PubChem (69509341) PubChem (70803584) PubChem (70859471) PubChem (71296126) PubChem (447865) PubChem (57809061) PubChem (59949925) PubChem (60054308) PubChem (70332686) PubChem (90899523) PubChem (4622926) PubChem (91266816) PubChem (91328992) PubChem (91370579) PubChem (91549642) PubChem (90829828) PubChem (90784642) PubChem (91613786) PubChem (71127686) PubChem (6520114) PubChem (122404663) PubChem (6166793) PubChem (6440032) PubChem (10184966) PubChem (6918680) PubChem (9891806) PubChem (123278527) PubChem (11225621) PubChem (12041744) PubChem (15331368) PubChem (125463315) PubChem (126456220) PubChem (133688255) PubChem (140436510) PubChem (154825564) PubChem (169410170) PubChem (171137682) PubChem (171666567)
12	W (F)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP) PubChem (4799) PubChem (46936826) PubChem (91532) PubChem (91885473) PubChem (157749842) PubChem (134943439) PubChem (157749839)

Sequence modifications

E: 5 - 145 (UniProt: P63042)

PDB	External Database	Details
Met 3	-	initiating methionine
Ala 4	-	expression tag

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	261631.3
Non-Polymers*	Number of molecules	18
	Total formula weight	3717.4
All*	Total formula weight	265348.7

*Water molecules are not included.