7DAD

EPD in complex with tubulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	105.163, 158.506, 181.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.907 - 2.850
R-factor	0.1799
Rwork	0.179
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i55
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.849	7.730	2.850
Rmerge	0.206	0.073	1.009
Rmeas	0.214	0.076	1.050
Rpim	0.058	0.021	0.288
Number of reflections	71614	3852	3523
<I/σ(I)>	3.2
Completeness [%]	100.0	100	100
Redundancy	13.3	12.3	12.9
CC(1/2)		0.997	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	277	6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7