7DA2

The crystal structure of the chicken FANCM-MHF complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Centromere protein S	polymer	107	11875.3	2	UniProt (E1BSW7) Pfam (PF15630)	Gallus gallus (Chicken)	CENP-S
2	C, D (B, D)	Centromere protein X	polymer	81	9361.7	2	UniProt (P0DJH7) Pfam (PF09415)	Gallus gallus (Chicken)	CENP-X
3	E (E)	Fanconi anemia group M protein	polymer	148	17604.8	1	UniProt (A0A1D5PRR9) Pfam (PF16783)	Gallus gallus (Chicken)	Protein FACM,ATP-dependent RNA helicase FANCM,Fanconi anemia-associated polypeptide of 250 kDa,FAAP250,Protein Hef ortholog
4	F, G, H, I, J (A, C, B, D, E)	water	water		18.0	47	Chemie (HOH)

Sequence modifications

A, C: 5 - 109 (UniProt: E1BSW7)

PDB	External Database	Details
Gly 3	-	expression tag
Ser 4	-	expression tag
Ala 29	Cys 26	engineered mutation
Ala 31	Cys 28	engineered mutation
Ala 58	Cys 55	engineered mutation

B, D: 4 - 82 (UniProt: P0DJH7)

PDB	External Database	Details
Gly 2	-	expression tag
Tyr 3	-	expression tag

E: 660 - 804 (UniProt: A0A1D5PRR9)

PDB	External Database	Details
Gly 658	-	expression tag
Arg 659	-	expression tag
Pro 805	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	60079.0
All*	Total formula weight	60079.0

*Water molecules are not included.