7DA2

The crystal structure of the chicken FANCM-MHF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	71.249, 78.469, 87.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.170 - 2.790
R-factor	0.2183
Rwork	0.216
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b0b
RMSD bond length	0.003
RMSD bond angle	0.527
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.170	2.890
High resolution limit [Å]	2.790	2.790
Rmerge	0.133	0.788
Rmeas	0.145	0.873
Number of reflections	12641	1232
<I/σ(I)>	32	4.32
Completeness [%]	99.3
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-Bicine pH 8.5, 0.1 M Carboxylic acids mix, 12.5% MPD, 12.5% PEG1000, 12.5% PEG3350