7D8X
CryoEM structure of human gamma-secretase in complex with E2012 and L685458
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Nicastrin | polymer | 709 | 78483.6 | 1 | UniProt (Q92542) Pfam (PF18266) Pfam (PF05450) In PDB | Homo sapiens (Human) | |
2 | B | Presenilin-1 | polymer | 467 | 52713.5 | 1 | UniProt (P49768) Pfam (PF01080) In PDB | Homo sapiens (Human) | PS-1,Protein S182 |
3 | C | Gamma-secretase subunit APH-1A | polymer | 265 | 29017.9 | 1 | UniProt (Q96BI3) Pfam (PF06105) In PDB | Homo sapiens (Human) | APH-1a,Aph-1alpha,Presenilin-stabilization factor |
4 | D | Gamma-secretase subunit PEN-2 | polymer | 143 | 16498.7 | 1 | UniProt (Q9NZ42) Pfam (PF10251) In PDB | Homo sapiens (Human) | Presenilin enhancer protein 2 |
5 | E, G, H, I, J | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 5 | In PDB GlyTouCan (G42666HT) | |||
6 | F | beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G89225LT) | |||
7 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) | |||
8 | B, C | 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 790.1 | 2 | Chemie (PC1) | |||
9 | B | 1-[(1S)-1-(4-fluorophenyl)ethyl]-3-[[3-methoxy-4-(4-methylimidazol-1-yl)phenyl]methylidene]piperidin-2-one | non-polymer | 419.5 | 1 | Chemie (GZR) | |||
10 | B | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 1 | Chemie (PTY) | |||
11 | B | ~{tert}-butyl ~{N}-[(2~{S},3~{R},5~{R})-6-[[(2~{S})-1-[[(2~{S})-1-azanyl-1-oxidanylidene-3-phenyl-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-3-oxidanyl-6-oxidanylidene-1-phenyl-5-(phenylmethyl)hexan-2-yl]carbamate | non-polymer | 672.9 | 1 | Chemie (FTO) | |||
12 | C | CHOLESTEROL | non-polymer | 386.7 | 3 | Chemie (CLR) |
Sequence modifications
D: 2 - 101 (UniProt: Q9NZ42)
PDB | External Database | Details |
---|---|---|
Met -41 | - | initiating methionine |
Ala -40 | - | expression tag |
Ser -39 | - | expression tag |
Trp -38 | - | expression tag |
Ser -37 | - | expression tag |
His -36 | - | expression tag |
Pro -35 | - | expression tag |
Gln -34 | - | expression tag |
Phe -33 | - | expression tag |
Glu -32 | - | expression tag |
Lys -31 | - | expression tag |
Gly -30 | - | expression tag |
Gly -29 | - | expression tag |
Gly -28 | - | expression tag |
Ala -27 | - | expression tag |
Arg -26 | - | expression tag |
Gly -25 | - | expression tag |
Gly -24 | - | expression tag |
Ser -23 | - | expression tag |
Gly -22 | - | expression tag |
Gly -21 | - | expression tag |
Gly -20 | - | expression tag |
Ser -19 | - | expression tag |
Trp -18 | - | expression tag |
Ser -17 | - | expression tag |
His -16 | - | expression tag |
Pro -15 | - | expression tag |
Gln -14 | - | expression tag |
Phe -13 | - | expression tag |
Glu -12 | - | expression tag |
Lys -11 | - | expression tag |
Gly -10 | - | expression tag |
Phe -9 | - | expression tag |
Asp -8 | - | expression tag |
Tyr -7 | - | expression tag |
Lys -6 | - | expression tag |
Asp -5 | - | expression tag |
Asp -4 | - | expression tag |
Asp -3 | - | expression tag |
Asp -2 | - | expression tag |
Lys -1 | - | expression tag |
Gly 0 | - | expression tag |
Thr 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 176713.7 | |
Branched | Number of molecules | 6 |
Total formula weight | 3032.8 | |
Non-Polymers* | Number of molecules | 14 |
Total formula weight | 5893.9 | |
All* | Total formula weight | 185640.4 |