7D7S

HIV-1 SF2 Nef in complex with the Fyn SH3 R96I mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Protein Nef	polymer	223	25585.8	2	UniProt (P03407) Pfam (PF00469) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate ARV2/SF2) (HIV-1)	3'ORF,Negative factor,F-protein
2	C, D	Tyrosine-protein kinase Fyn	polymer	72	8255.0	2	UniProt (E5RFS5) Pfam (PF00018) In PDB	Homo sapiens (Human)

Sequence modifications

A, B: 1 - 209 (UniProt: P03407)

PDB	External Database	Details
Ser 210	-	expression tag
Lys 211	-	expression tag
Leu 212	-	expression tag
Ala 213	-	expression tag
Ala 214	-	expression tag
Ala 215	-	expression tag
Leu 216	-	expression tag
Glu 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag

C, D: 82 - 144 (UniProt: E5RFS5)

PDB	External Database	Details
Met 81	-	initiating methionine
Ile 96	Arg 96	engineered mutation
Leu 145	-	expression tag
Glu 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	67681.6
All*	Total formula weight	67681.6

*Water molecules are not included.