7CZ5
Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | R | Growth hormone-releasing hormone receptor,growth hormone-releasing hormone receptor | polymer | 556 | 61651.4 | 1 | UniProt (Q02643) Pfam (PF02793) Pfam (PF00002) In PDB | Homo sapiens (Human) | GHRH receptor,Growth hormone-releasing factor receptor,GRFR |
2 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
3 | P | Somatoliberin | polymer | 44 | 5048.7 | 1 | UniProt (P01286) Pfam (PF00123) In PDB | Homo sapiens | Growth hormone-releasing factor,GRF,Growth hormone-releasing hormone,GHRH,Somatocrinin,Somatorelin |
4 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 400 | 43706.8 | 1 | UniProt (P54311) Pfam (PF00400) In PDB | Rattus norvegicus (Rat) | Transducin beta chain 1 |
5 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) In PDB | Bos taurus (Bovine) | G gamma-I |
6 | N | Nanobody35 | polymer | 126 | 13711.3 | 1 | synthetic construct | ||
7 | R, A | PALMITIC ACID | non-polymer | 256.4 | 2 | Chemie (PLM) | |||
8 | R | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
9 | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
R: 23 - 405 (UniProt: Q02643)
R: 406 - 578 (PDB: 7CZ5)
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P54311)
R: 406 - 578 (PDB: 7CZ5)
A: 1 - 394 (UniProt: P63092)
PDB | External Database | Details |
---|---|---|
Asn 54 | Ser 54 | engineered mutation |
Ala 226 | Gly 226 | engineered mutation |
Ala 268 | Glu 268 | engineered mutation |
Lys 271 | Asn 271 | engineered mutation |
Asp 274 | Lys 274 | engineered mutation |
Lys 280 | Arg 280 | engineered mutation |
Asp 284 | Thr 284 | engineered mutation |
Thr 285 | Ile 285 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -33 | - | initiating methionine |
His -32 | - | expression tag |
His -31 | - | expression tag |
His -30 | - | expression tag |
His -29 | - | expression tag |
His -28 | - | expression tag |
His -27 | - | expression tag |
Ser -26 | - | expression tag |
Ser -25 | - | expression tag |
Gly -24 | - | expression tag |
Leu -23 | - | expression tag |
Val -22 | - | expression tag |
Pro -21 | - | expression tag |
Arg -20 | - | expression tag |
Gly -19 | - | expression tag |
Ser -18 | - | expression tag |
His -17 | - | expression tag |
Met -16 | - | expression tag |
Ala -15 | - | expression tag |
Ser -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Gly 341 | - | expression tag |
Ser 342 | - | expression tag |
Ser 343 | - | expression tag |
Gly 344 | - | expression tag |
Gly 345 | - | expression tag |
Gly 346 | - | expression tag |
Gly 347 | - | expression tag |
Ser 348 | - | expression tag |
Gly 349 | - | expression tag |
Gly 350 | - | expression tag |
Gly 351 | - | expression tag |
Gly 352 | - | expression tag |
Ser 353 | - | expression tag |
Ser 354 | - | expression tag |
Gly 355 | - | expression tag |
Val 356 | - | expression tag |
Ser 357 | - | expression tag |
Gly 358 | - | expression tag |
Trp 359 | - | expression tag |
Arg 360 | - | expression tag |
Leu 361 | - | expression tag |
Phe 362 | - | expression tag |
Lys 363 | - | expression tag |
Lys 364 | - | expression tag |
Ile 365 | - | expression tag |
Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 177662.8 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 899.5 | |
All* | Total formula weight | 178562.3 |