7CZ5

Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Growth hormone-releasing hormone receptor,growth hormone-releasing hormone receptor	polymer	556	61651.4	1	UniProt (Q02643) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (Human)	GHRH receptor,Growth hormone-releasing factor receptor,GRFR
2	B (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
3	C (P)	Somatoliberin	polymer	44	5048.7	1	UniProt (P01286) Pfam (PF00123)	Homo sapiens	Growth hormone-releasing factor,GRF,Growth hormone-releasing hormone,GHRH,Somatocrinin,Somatorelin
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	400	43706.8	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Rat)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
6	F (N)	Nanobody35	polymer	126	13711.3	1		synthetic construct
7	G, I (R, A)	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)
8	H (R)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)
9	J, K, L (R, A, P)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

R: 23 - 405 (UniProt: Q02643)
R: 406 - 578 (PDB: 7CZ5)
A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -33	-	initiating methionine
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Leu -23	-	expression tag
Val -22	-	expression tag
Pro -21	-	expression tag
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Met -16	-	expression tag
Ala -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	177662.8
Non-Polymers*	Number of molecules	3
	Total formula weight	899.5
All*	Total formula weight	178562.3

*Water molecules are not included.