7CYQ

Cryo-EM structure of an extended SARS-CoV-2 replication and transcription complex reveals an intermediate state in cap synthesis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RNA-directed RNA polymerase	polymer	942	108162.5	1	UniProt (P0DTD1) Pfam (PF06478) Pfam (PF00680) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,Pol,RdRp,Non-structural protein 12,nsp12,Replicase polyprotein 1ab
2	B, D	Non-structural protein 8	polymer	198	21903.0	2	UniProt (P0DTD1) Pfam (PF08717) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp8
3	C	Non-structural protein 7	polymer	83	9248.8	1	UniProt (P0DTD1) Pfam (PF08716) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp7
4	I	Primer	polymer	33	10704.4	1		Severe acute respiratory syndrome coronavirus 2
5	J	Template	polymer	59	18808.2	1		Severe acute respiratory syndrome coronavirus 2
6	F, E	Helicase	polymer	601	66930.5	2	UniProt (P0DTD1) Pfam (PF20632) Pfam (PF20633) Pfam (PF20631) Pfam (PF13087) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,Hel,Non-structural protein 13,nsp13,Replicase polyprotein 1ab
7	G	Non-structural protein 9	polymer	117	12794.6	1	UniProt (P0DTD1) Pfam (PF08710) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp9,Replicase polyprotein 1ab
8	A, F, E	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
9	A	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
10	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 932 (UniProt: P0DTD1)

PDB	External Database	Details
His 933	-	expression tag
His 934	-	expression tag
His 935	-	expression tag
His 936	-	expression tag
His 937	-	expression tag
His 938	-	expression tag
His 939	-	expression tag
His 940	-	expression tag
His 941	-	expression tag
His 942	-	expression tag

G: 1 - 113 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -3	-	expression tag
Asn -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	337385.6
Non-Polymers*	Number of molecules	10
	Total formula weight	990.8
All*	Total formula weight	338376.4

*Water molecules are not included.