7CYQ
Cryo-EM structure of an extended SARS-CoV-2 replication and transcription complex reveals an intermediate state in cap synthesis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RNA-directed RNA polymerase | polymer | 942 | 108162.5 | 1 | UniProt (P0DTD1) Pfam (PF06478) Pfam (PF00680) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | pp1ab,ORF1ab polyprotein,Pol,RdRp,Non-structural protein 12,nsp12,Replicase polyprotein 1ab |
| 2 | B, D (B, D) | Non-structural protein 8 | polymer | 198 | 21903.0 | 2 | UniProt (P0DTD1) Pfam (PF08717) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | pp1ab,ORF1ab polyprotein,nsp8 |
| 3 | C (C) | Non-structural protein 7 | polymer | 83 | 9248.8 | 1 | UniProt (P0DTD1) Pfam (PF08716) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | pp1ab,ORF1ab polyprotein,nsp7 |
| 4 | E (I) | Primer | polymer | 33 | 10704.4 | 1 | Severe acute respiratory syndrome coronavirus 2 | ||
| 5 | F (J) | Template | polymer | 59 | 18808.2 | 1 | Severe acute respiratory syndrome coronavirus 2 | ||
| 6 | G, H (F, E) | Helicase | polymer | 601 | 66930.5 | 2 | UniProt (P0DTD1) Pfam (PF20632) Pfam (PF20633) Pfam (PF20631) Pfam (PF13087) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | pp1ab,ORF1ab polyprotein,Hel,Non-structural protein 13,nsp13,Replicase polyprotein 1ab |
| 7 | I (G) | Non-structural protein 9 | polymer | 117 | 12794.6 | 1 | UniProt (P0DTD1) Pfam (PF08710) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | pp1ab,ORF1ab polyprotein,nsp9,Replicase polyprotein 1ab |
| 8 | J, K, N, O, P... (A, F, E) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 9 | L (A) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 10 | M (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 1 - 932 (UniProt: P0DTD1)
G: 1 - 113 (UniProt: P0DTD1)
| PDB | External Database | Details |
|---|---|---|
| His 933 | - | expression tag |
| His 934 | - | expression tag |
| His 935 | - | expression tag |
| His 936 | - | expression tag |
| His 937 | - | expression tag |
| His 938 | - | expression tag |
| His 939 | - | expression tag |
| His 940 | - | expression tag |
| His 941 | - | expression tag |
| His 942 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -3 | - | expression tag |
| Asn -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Met 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 337385.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 990.8 | |
| All* | Total formula weight | 338376.4 |
