7CX3
Cryo-EM structure of the Taprenepag-bound EP2-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | R | Prostaglandin E2 receptor EP2 subtype | polymer | 358 | 39800.4 | 1 | UniProt (P43116) Pfam (PF00001) In PDB | Homo sapiens (Human) | PGE2 receptor EP2 subtype,Prostanoid EP2 receptor |
2 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
3 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39489.2 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
4 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
5 | N | Nanobody-35 | polymer | 128 | 13885.4 | 1 | synthetic construct | ||
6 | R | 2-[3-[[(4-pyrazol-1-ylphenyl)methyl-pyridin-3-ylsulfonyl-amino]methyl]phenoxy]ethanoic acid | non-polymer | 478.5 | 1 | Chemie (GNO) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Asn 54 | Ser 54 | engineered mutation |
Ala 226 | Gly 226 | engineered mutation |
Ala 268 | Glu 268 | engineered mutation |
Lys 271 | Asn 271 | engineered mutation |
Asp 274 | Lys 274 | engineered mutation |
Lys 280 | Arg 280 | engineered mutation |
Asp 284 | Thr 284 | engineered mutation |
Thr 285 | Ile 285 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -17 | - | initiating methionine |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 146719.6 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 478.5 | |
All* | Total formula weight | 147198.1 |