7CR6

Synechocystis Cas1-Cas2/prespacer binary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	CRISPR-associated endonuclease Cas1	polymer	336	37811.2	4	UniProt (Q6ZEI2) Pfam (PF01867) In PDB	Synechocystis sp. (strain PCC 6803 / Kazusa)
2	E, F	CRISPR-associated endoribonuclease Cas2 1	polymer	105	11656.4	2	UniProt (Q6ZEI1) Pfam (PF09827) In PDB	Synechocystis sp. (strain PCC 6803 / Kazusa)
3	G	DNA (36-MER)	polymer	36	10961.0	1		synthetic construct
4	H	DNA (36-MER)	polymer	36	11064.1	1		synthetic construct
5		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 325 (UniProt: Q6ZEI2)

PDB	External Database	Details
Gly -10	-	expression tag
Ala -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Thr -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

E, F: 1 - 94 (UniProt: Q6ZEI1)

PDB	External Database	Details
Gly -10	-	expression tag
Ala -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Thr -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	196582.9
All*	Total formula weight	196582.9

*Water molecules are not included.