7CR6

Synechocystis Cas1-Cas2/prespacer binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	125.556, 215.160, 191.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.810 - 3.720
R-factor	0.278
Rwork	0.276
R-free	0.32420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zpw
RMSD bond length	0.003
RMSD bond angle	0.670
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.790
High resolution limit [Å]	3.700	3.700
Number of reflections	27252	1782
<I/σ(I)>	11
Completeness [%]	99.4
Redundancy	12.5
CC(1/2)	0.983	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% MPD, 0.1 M imidazole pH 6.5, 0.2 M (NH4)2SO4, and 10% (w/v) PEG3350