7CQI
Cryo-EM structure of the substrate-bound SPT-ORMDL3 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (S, C) | Serine palmitoyltransferase 1 | polymer | 473 | 52806.8 | 2 | UniProt (O15269) Pfam (PF00155) | Homo sapiens (Human) | Long chain base biosynthesis protein 1,LCB 1,Serine-palmitoyl-CoA transferase 1,SPT1 |
| 2 | B (T) | Serine palmitoyltransferase 2 | polymer | 562 | 63004.2 | 1 | UniProt (O15270) Pfam (PF00155) | Homo sapiens (Human) | Long chain base biosynthesis protein 2,LCB 2,Long chain base biosynthesis protein 2a,LCB2a,Serine-palmitoyl-CoA transferase 2,SPT 2 |
| 3 | C (A) | ORM1-like protein 3 | polymer | 153 | 17512.6 | 1 | UniProt (Q8N138) Pfam (PF04061) | Homo sapiens (Human) | |
| 4 | D (E) | Serine palmitoyltransferase small subunit A | polymer | 92 | 10742.4 | 1 | UniProt (Q969W0) Pfam (PF11779) | Homo sapiens (Human) | Small subunit of serine palmitoyltransferase A,ssSPTa |
| 5 | F (T) | [3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE | non-polymer | 336.2 | 1 | Chemie (PLS) | |||
| 6 | G (T) | [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3R)-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-4-[[3-oxidanylidene-3-[2-(2-oxidanylideneheptadecylsulfanyl)ethylamino]propyl]amino]butyl] hydrogen phosphate | non-polymer | 1020.0 | 1 | Chemie (GE0) |
Sequence modifications
E: 1 - 71 (UniProt: Q969W0)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | initiating methionine |
| Ala -19 | - | expression tag |
| Asp -18 | - | expression tag |
| Tyr -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Asp -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Lys -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Pro -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Arg 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 196872.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1356.2 | |
| All* | Total formula weight | 198228.9 |
