7CN4

Cryo-EM structure of bat RaTG13 spike glycoprotein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike glycoprotein	polymer	1267	140512.7	3	UniProt (A0A6B9WHD3) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) UniProt (by SIFTS) (P0DTC2) In PDB	Bat coronavirus RaTG13
2	D, E, F, G, H...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	9	In PDB GlyTouCan (G42666HT)
3	B, C, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	36	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 1209 (UniProt: A0A6B9WHD3)

PDB	External Database	Details
Pro 982	Lys 982	engineered mutation
Pro 983	Val 983	engineered mutation
Leu 1210	-	expression tag
Glu 1211	-	expression tag
Val 1212	-	expression tag
Leu 1213	-	expression tag
Phe 1214	-	expression tag
Gln 1215	-	expression tag
Gly 1216	-	expression tag
Pro 1217	-	expression tag
Gly 1218	-	expression tag
Gly 1219	-	expression tag
Gly 1220	-	expression tag
Ser 1221	-	expression tag
Gly 1222	-	expression tag
Gly 1223	-	expression tag
Gly 1224	-	expression tag
Ser 1225	-	expression tag
Gly 1226	-	expression tag
Tyr 1227	-	expression tag
Ile 1228	-	expression tag
Pro 1229	-	expression tag
Glu 1230	-	expression tag
Ala 1231	-	expression tag
Pro 1232	-	expression tag
Arg 1233	-	expression tag
Asp 1234	-	expression tag
Gly 1235	-	expression tag
Gln 1236	-	expression tag
Ala 1237	-	expression tag
Tyr 1238	-	expression tag
Val 1239	-	expression tag
Arg 1240	-	expression tag
Lys 1241	-	expression tag
Asp 1242	-	expression tag
Gly 1243	-	expression tag
Glu 1244	-	expression tag
Trp 1245	-	expression tag
Val 1246	-	expression tag
Leu 1247	-	expression tag
Leu 1248	-	expression tag
Ser 1249	-	expression tag
Thr 1250	-	expression tag
Phe 1251	-	expression tag
Leu 1252	-	expression tag
Gly 1253	-	expression tag
His 1254	-	expression tag
His 1255	-	expression tag
His 1256	-	expression tag
His 1257	-	expression tag
His 1258	-	expression tag
His 1259	-	expression tag
Trp 1260	-	expression tag
Ser 1261	-	expression tag
His 1262	-	expression tag
Pro 1263	-	expression tag
Gln 1264	-	expression tag
Phe 1265	-	expression tag
Glu 1266	-	expression tag
Lys 1267	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	421538.2
Branched	Number of molecules	9
	Total formula weight	3819.6
Non-Polymers*	Number of molecules	36
	Total formula weight	7963.5
All*	Total formula weight	433321.3

*Water molecules are not included.