7CMU

Dopamine Receptor D3R-Gi-Pramipexole complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	E	scfv16	polymer	248	26466.5	1		Mus musculus (Mouse)
5	R	Soluble cytochrome b562,D(3) dopamine receptor	polymer	545	60816.8	1	UniProt (P0ABE7) UniProt (P35462) Pfam (PF07361) Pfam (PF00001) In PDB	Escherichia coli	Cytochrome b-562,Dopamine D3 receptor
6	R	(6S)-N6-propyl-4,5,6,7-tetrahydro-1,3-benzothiazole-2,6-diamine	non-polymer		211.3	1	Chemie (G6L)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: -116 - -11 (UniProt: P0ABE7)

PDB	External Database	Details
Asp -144	-	expression tag
Tyr -143	-	expression tag
Lys -142	-	expression tag
Asp -141	-	expression tag
Asp -140	-	expression tag
Asp -139	-	expression tag
Asp -138	-	expression tag
Ala -137	-	expression tag
Lys -136	-	expression tag
Leu -135	-	expression tag
Gln -134	-	expression tag
Thr -133	-	expression tag
Met -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
Trp -110	Met 29	engineered mutation
Ile -15	His 124	engineered mutation
Leu -11	Arg 128	engineered mutation
Ala -10	-	linker
Ser -9	-	linker
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Gly -1	-	linker
Thr 0	-	linker

R: 1 - 400 (UniProt: P35462)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	173474.0
Non-Polymers*	Number of molecules	1
	Total formula weight	211.3
All*	Total formula weight	173685.3

*Water molecules are not included.