7CL8

Testosterone-bound structure of CYP154C2 from Streptomyces avermitilis in an closed conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cytochrome P450 hydroxylase	polymer	420	45272.4	1	UniProt (Q82GL5) Pfam (PF00067) In PDB	Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)	CYP154C2
2	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
5	A	TESTOSTERONE	non-polymer		288.4	1	Chemie (TES)
6		water	water		18.0	640	Chemie (HOH)

Sequence modifications

A: 1 - 400 (UniProt: Q82GL5)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45272.4
Non-Polymers*	Number of molecules	5
	Total formula weight	1267.3
All*	Total formula weight	46539.7

*Water molecules are not included.