7CKY
Cryo-EM structure of PW0464 bound dopamine receptor DRD1-Gs signaling complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45769.6 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 348 | 38257.8 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 4 | D (N) | Nanobody 35 | polymer | 156 | 16926.1 | 1 | Lama glama | ||
| 5 | E (R) | D(1A) dopamine receptor | polymer | 446 | 49339.2 | 1 | UniProt (P21728) Pfam (PF00001) | Homo sapiens (Human) | Dopamine D1 receptor |
| 6 | F (R) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
| 7 | G (R) | 6-[4-[3-[bis(fluoranyl)methoxy]pyridin-2-yl]oxy-2-methyl-phenyl]-1,5-dimethyl-pyrimidine-2,4-dione | non-polymer | 389.4 | 1 | Chemie (G3U) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Thr 205 | Ser 205 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 158153.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 776.0 | |
| All* | Total formula weight | 158929.7 |
