7CKQ
The cryo-EM structure of B. subtilis BmrR transcription activation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 314 | 34842.4 | 2 | UniProt (P20429) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Bacillus subtilis (strain 168) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1193 | 133847.9 | 1 | UniProt (P37870) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Bacillus subtilis (strain 168) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1199 | 134445.0 | 1 | UniProt (P37871) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Bacillus subtilis (strain 168) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E | DNA-directed RNA polymerase subunit omega | polymer | 67 | 7766.9 | 1 | UniProt (O35011) Pfam (PF01192) In PDB | Bacillus subtilis (strain 168) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F | RNA polymerase sigma factor SigA | polymer | 371 | 43007.4 | 1 | UniProt (P06224) Pfam (PF03979) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Bacillus subtilis (strain 168) | Sigma-43,Sigma-A |
| 6 | 1 | DNA (50-MER) | polymer | 50 | 15489.9 | 1 | synthetic construct | ||
| 7 | 2 | DNA (50-MER) | polymer | 50 | 15485.9 | 1 | synthetic construct | ||
| 8 | G, I | Multidrug-efflux transporter 1 regulator | polymer | 282 | 32951.7 | 2 | UniProt (P39075) Pfam (PF00376) Pfam (PF06445) In PDB | Bacillus subtilis (strain 168) | |
| 9 | H | UPF0356 protein YkzG | polymer | 69 | 8263.4 | 1 | UniProt (O31718) Pfam (PF07288) In PDB | Bacillus subtilis (strain 168) | |
| 10 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 12 | G, I | TETRAPHENYLPHOSPHONIUM | non-polymer | 339.4 | 2 | Chemie (P4P) |
Sequence modifications
G, I: 1 - 278 (UniProt: P39075)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 493894.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 833.9 | |
| All* | Total formula weight | 494728.5 |
