7CJ9

Crystal structure of N-terminal His-tagged D-allulose 3-epimerase from Methylomonas sp. with additional C-terminal residues

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Epimerase	polymer	316	34485.9	8	UniProt (A0A172U6X0) Pfam (PF01261) In PDB	Methylomonas sp. DH-1
2	F, G, A, H, B...	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
3	E, F, G, A, H...	D-fructose	non-polymer		180.2	8	Chemie (FUD)
4	E, F, G, A, H...	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	8	Chemie (EPE)
5	F, C, D, E	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
6		water	water		18.0	2158	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 286 (UniProt: A0A172U6X0)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag
Ile 9	Tyr 9	engineered mutation
Phe 37	Tyr 37	engineered mutation
Leu 286	Tyr 286	engineered mutation
Thr 287	-	expression tag
Ala 288	-	expression tag
Ile 289	-	expression tag
Lys 290	-	expression tag
Thr 291	-	expression tag
Ile 292	-	expression tag
Glu 293	-	expression tag
Leu 294	-	expression tag
His 295	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	275886.8
Non-Polymers*	Number of molecules	31
	Total formula weight	4221.7
All*	Total formula weight	280108.5

*Water molecules are not included.