7CJ9
Crystal structure of N-terminal His-tagged D-allulose 3-epimerase from Methylomonas sp. with additional C-terminal residues
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Epimerase | polymer | 316 | 34485.9 | 8 | UniProt (A0A172U6X0) Pfam (PF01261) | Methylomonas sp. DH-1 | |
| 2 | EA, HA, I, KA, L... (F, G, A, H, B...) | MANGANESE (II) ION | non-polymer | 54.9 | 8 | Chemie (MN) | |||
| 3 | AA, FA, IA, J, LA... (E, F, G, A, H...) | D-fructose | non-polymer | 180.2 | 8 | Chemie (FUD) | |||
| 4 | BA, GA, JA, K, MA... (E, F, G, A, H...) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 8 | Chemie (EPE) | |||
| 5 | CA, DA, O, P, Q... (F, C, D, E) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 7 | Chemie (EDO) | |||
| 6 | NA, OA, PA, QA, RA... (A, B, C, D, E...) | water | water | 18.0 | 2158 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 1 - 286 (UniProt: A0A172U6X0)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | initiating methionine |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Arg -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ile 9 | Tyr 9 | engineered mutation |
| Phe 37 | Tyr 37 | engineered mutation |
| Leu 286 | Tyr 286 | engineered mutation |
| Thr 287 | - | expression tag |
| Ala 288 | - | expression tag |
| Ile 289 | - | expression tag |
| Lys 290 | - | expression tag |
| Thr 291 | - | expression tag |
| Ile 292 | - | expression tag |
| Glu 293 | - | expression tag |
| Leu 294 | - | expression tag |
| His 295 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 275886.8 | |
| Non-Polymers* | Number of molecules | 31 |
| Total formula weight | 4221.7 | |
| All* | Total formula weight | 280108.5 |
