Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7CJ9

Crystal structure of N-terminal His-tagged D-allulose 3-epimerase from Methylomonas sp. with additional C-terminal residues

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016853	molecular_function	isomerase activity
A	0046872	molecular_function	metal ion binding
B	0016853	molecular_function	isomerase activity
B	0046872	molecular_function	metal ion binding
C	0016853	molecular_function	isomerase activity
C	0046872	molecular_function	metal ion binding
D	0016853	molecular_function	isomerase activity
D	0046872	molecular_function	metal ion binding
E	0016853	molecular_function	isomerase activity
E	0046872	molecular_function	metal ion binding
F	0016853	molecular_function	isomerase activity
F	0046872	molecular_function	metal ion binding
G	0016853	molecular_function	isomerase activity
G	0046872	molecular_function	metal ion binding
H	0016853	molecular_function	isomerase activity
H	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue MN A 301

Chain	Residue
A	GLU152
A	ASP185
A	HIS211
A	GLU246
A	FUD302

site_id	AC2
Number of Residues	15
Details	binding site for residue FUD A 302

Chain	Residue
A	GLY110
A	VAL111
A	GLU152
A	GLU158
A	ASP185
A	HIS188
A	HIS211
A	ARG217
A	GLU246
A	LEU261
A	MN301
A	HIS12
A	SER69
A	LEU70
A	GLY71

site_id	AC3
Number of Residues	9
Details	binding site for residue EPE A 303

Chain	Residue
A	ARG128
A	ASN160
A	ILE161
A	ASN163
A	GLU167
A	HOH541
B	TRP264
B	ARG265
C	LYS27

site_id	AC4
Number of Residues	5
Details	binding site for residue MN B 301

Chain	Residue
B	GLU152
B	ASP185
B	HIS211
B	GLU246
B	FUD302

site_id	AC5
Number of Residues	13
Details	binding site for residue FUD B 302

Chain	Residue
B	HIS12
B	SER69
B	LEU70
B	VAL111
B	GLU152
B	GLU158
B	ASP185
B	HIS188
B	HIS211
B	ARG217
B	GLU246
B	LEU261
B	MN301

site_id	AC6
Number of Residues	9
Details	binding site for residue EPE B 303

Chain	Residue
A	TRP264
A	ARG265
B	ARG125
B	ASN160
B	ILE161
B	ASN163
B	GLU167
B	HOH409
D	LYS27

site_id	AC7
Number of Residues	5
Details	binding site for residue EDO C 301

Chain	Residue
C	ALA197
C	SER229
C	ALA232
C	ALA233
C	GLN236

site_id	AC8
Number of Residues	6
Details	binding site for residue EDO C 302

Chain	Residue
C	ALA115
C	GLY117
C	LYS118
C	TYR119
C	HOH408
C	HOH454

site_id	AC9
Number of Residues	3
Details	binding site for residue EDO C 303

Chain	Residue
C	LEU7
C	ASP38
C	LYS283

site_id	AD1
Number of Residues	5
Details	binding site for residue MN C 304

Chain	Residue
C	GLU152
C	ASP185
C	HIS211
C	GLU246
C	FUD305

site_id	AD2
Number of Residues	12
Details	binding site for residue FUD C 305

Chain	Residue
C	HIS12
C	SER69
C	VAL111
C	GLU152
C	GLU158
C	ASP185
C	HIS188
C	HIS211
C	ARG217
C	GLU246
C	LEU261
C	MN304

site_id	AD3
Number of Residues	8
Details	binding site for residue EPE C 306

Chain	Residue
A	LYS27
C	ARG128
C	ASN160
C	ILE161
C	ASN163
C	GLU167
D	TRP264
D	ARG265

site_id	AD4
Number of Residues	4
Details	binding site for residue EDO D 301

Chain	Residue
D	GLY117
D	LYS118
D	TYR119
D	HOH462

site_id	AD5
Number of Residues	5
Details	binding site for residue MN D 302

Chain	Residue
D	GLU152
D	ASP185
D	HIS211
D	GLU246
D	FUD303

site_id	AD6
Number of Residues	13
Details	binding site for residue FUD D 303

Chain	Residue
D	HIS12
D	GLU41
D	SER69
D	VAL111
D	GLU152
D	GLU158
D	ASP185
D	HIS188
D	HIS211
D	ARG217
D	GLU246
D	LEU261
D	MN302

site_id	AD7
Number of Residues	10
Details	binding site for residue EPE D 304

Chain	Residue
B	LYS27
C	TRP264
C	ARG265
D	ARG128
D	ASN160
D	ILE161
D	ASN163
D	GLU167
D	HOH497
D	HOH551

site_id	AD8
Number of Residues	4
Details	binding site for residue EDO E 301

Chain	Residue
E	ALA115
E	GLY117
E	LYS118
E	HOH476

site_id	AD9
Number of Residues	5
Details	binding site for residue MN E 302

Chain	Residue
E	GLU152
E	ASP185
E	HIS211
E	GLU246
E	FUD303

site_id	AE1
Number of Residues	14
Details	binding site for residue FUD E 303

Chain	Residue
E	HIS12
E	SER69
E	LEU70
E	GLY110
E	VAL111
E	GLU152
E	GLU158
E	ASP185
E	HIS188
E	HIS211
E	ARG217
E	GLU246
E	LEU261
E	MN302

site_id	AE2
Number of Residues	10
Details	binding site for residue EPE E 304

Chain	Residue
E	ARG128
E	ASN160
E	ILE161
E	ASN163
E	GLU167
E	HOH449
E	HOH548
F	TRP264
F	ARG265
G	LYS27

site_id	AE3
Number of Residues	5
Details	binding site for residue EDO F 301

Chain	Residue
F	LEU45
F	THR76
F	LEU92
F	HOH414
F	HOH541

site_id	AE4
Number of Residues	7
Details	binding site for residue EDO F 302

Chain	Residue
F	ALA74
F	GLY117
F	LYS118
F	TYR119
F	HOH406
F	HOH415
F	HOH495

site_id	AE5
Number of Residues	5
Details	binding site for residue MN F 303

Chain	Residue
F	GLU152
F	ASP185
F	HIS211
F	GLU246
F	FUD304

site_id	AE6
Number of Residues	13
Details	binding site for residue FUD F 304

Chain	Residue
F	HIS12
F	LEU45
F	SER69
F	VAL111
F	GLU152
F	GLU158
F	ASP185
F	HIS188
F	HIS211
F	ARG217
F	GLU246
F	LEU261
F	MN303

site_id	AE7
Number of Residues	10
Details	binding site for residue EPE F 305

Chain	Residue
E	TRP264
E	ARG265
F	ARG125
F	ARG128
F	ASN160
F	ILE161
F	ASN163
F	GLU167
F	HOH434
H	LYS27

site_id	AE8
Number of Residues	5
Details	binding site for residue MN G 301

Chain	Residue
G	GLU152
G	ASP185
G	HIS211
G	GLU246
G	FUD302

site_id	AE9
Number of Residues	15
Details	binding site for residue FUD G 302

Chain	Residue
G	HIS12
G	ALA43
G	SER69
G	LEU70
G	GLY110
G	VAL111
G	GLU152
G	GLU158
G	ASP185
G	HIS188
G	HIS211
G	ARG217
G	GLU246
G	LEU261
G	MN301

site_id	AF1
Number of Residues	8
Details	binding site for residue EPE G 303

Chain	Residue
E	LYS27
G	ASN160
G	ILE161
G	ASN163
G	GLU167
G	HOH414
H	TRP264
H	ARG265

site_id	AF2
Number of Residues	5
Details	binding site for residue MN H 301

Chain	Residue
H	GLU152
H	ASP185
H	HIS211
H	GLU246
H	FUD302

site_id	AF3
Number of Residues	15
Details	binding site for residue FUD H 302

Chain	Residue
H	HIS12
H	SER69
H	LEU70
H	GLY71
H	GLY110
H	VAL111
H	GLU152
H	GLU158
H	ASP185
H	HIS188
H	HIS211
H	ARG217
H	GLU246
H	LEU261
H	MN301

site_id	AF4
Number of Residues	10
Details	binding site for residue EPE H 303

Chain	Residue
F	LYS27
G	TRP264
G	ARG265
H	ARG128
H	ASN160
H	ILE161
H	ASN163
H	GLU167
H	HOH489
H	HOH501

Functional Information from PROSITE/UniProt

site_id	PS00105
Number of Residues	14
Details	AA_TRANSFER_CLASS_1 Aminotransferases class-I pyridoxal-phosphate attachment site. GMAKsvLAaGDRLG

Chain	Residue	Details
A	GLY195-GLY208

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	ACT_SITE: Proton donor/acceptor => ECO:0000250\|UniProtKB:Q9WYP7

Chain	Residue	Details
A	GLU152
E	GLU246
F	GLU152
F	GLU246
G	GLU152
G	GLU246
H	GLU152
H	GLU246
A	GLU246
B	GLU152
B	GLU246
C	GLU152
C	GLU246
D	GLU152
D	GLU246
E	GLU152

site_id	SWS_FT_FI2
Number of Residues	40
Details	BINDING: BINDING => ECO:0000269\|PubMed:33838083, ECO:0007744\|PDB:7CJ5, ECO:0007744\|PDB:7CJ9

Chain	Residue	Details
A	HIS12
B	ARG217
C	HIS12
C	SER69
C	GLU158
C	HIS188
C	ARG217
D	HIS12
D	SER69
D	GLU158
D	HIS188
A	SER69
D	ARG217
E	HIS12
E	SER69
E	GLU158
E	HIS188
E	ARG217
F	HIS12
F	SER69
F	GLU158
F	HIS188
A	GLU158
F	ARG217
G	HIS12
G	SER69
G	GLU158
G	HIS188
G	ARG217
H	HIS12
H	SER69
H	GLU158
H	HIS188
A	HIS188
H	ARG217
A	ARG217
B	HIS12
B	SER69
B	GLU158
B	HIS188

site_id	SWS_FT_FI3
Number of Residues	32
Details	BINDING: BINDING => ECO:0000269\|PubMed:33838083, ECO:0000312\|PDB:7CJ5, ECO:0007744\|PDB:7CJ4, ECO:0007744\|PDB:7CJ6, ECO:0007744\|PDB:7CJ7, ECO:0007744\|PDB:7CJ8, ECO:0007744\|PDB:7CJ9

Chain	Residue	Details
A	GLU152
C	ASP185
C	HIS211
C	GLU246
D	GLU152
D	ASP185
D	HIS211
D	GLU246
E	GLU152
E	ASP185
E	HIS211
A	ASP185
E	GLU246
F	GLU152
F	ASP185
F	HIS211
F	GLU246
G	GLU152
G	ASP185
G	HIS211
G	GLU246
H	GLU152
A	HIS211
H	ASP185
H	HIS211
H	GLU246
A	GLU246
B	GLU152
B	ASP185
B	HIS211
B	GLU246
C	GLU152

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)