7CJ8

Crystal structure of N-terminal His-tagged D-allulose 3-epimerase from Methylomonas sp. in complex with D-allulose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Epimerase	polymer	307	33592.7	6	UniProt (A0A172U6X0) Pfam (PF01261) In PDB	Methylomonas sp. DH-1
2	A, B, C, D, E...	MANGANESE (II) ION	non-polymer		54.9	6	Chemie (MN)
3	A, B, C, D, E...	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	6	Chemie (EPE)
4	A, B, C, D, E...	D-psicose	non-polymer		180.2	6	Chemie (PSJ)
5		water	water		18.0	939	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 286 (UniProt: A0A172U6X0)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	201556.1
Non-Polymers*	Number of molecules	18
	Total formula weight	2840.4
All*	Total formula weight	204396.5

*Water molecules are not included.