7CJ8

Crystal structure of N-terminal His-tagged D-allulose 3-epimerase from Methylomonas sp. in complex with D-allulose

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016853	molecular_function	isomerase activity
A	0046872	molecular_function	metal ion binding
B	0016853	molecular_function	isomerase activity
B	0046872	molecular_function	metal ion binding
C	0016853	molecular_function	isomerase activity
C	0046872	molecular_function	metal ion binding
D	0016853	molecular_function	isomerase activity
D	0046872	molecular_function	metal ion binding
E	0016853	molecular_function	isomerase activity
E	0046872	molecular_function	metal ion binding
F	0016853	molecular_function	isomerase activity
F	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue MN A 301

Chain	Residue
A	GLU152
A	ASP185
A	HIS211
A	GLU246
A	PSJ303

---

site_id	AC2
Number of Residues	9
Details	binding site for residue EPE A 302

Chain	Residue
A	GLU167
A	HOH491
B	TRP264
B	ARG265
D	LYS27
A	ARG128
A	ASN160
A	ILE161
A	ASN163

---

site_id	AC3
Number of Residues	12
Details	binding site for residue PSJ A 303

Chain	Residue
A	HIS12
A	VAL111
A	GLU152
A	GLU158
A	ASP185
A	HIS188
A	HIS211
A	ARG217
A	GLU246
A	LEU257
A	LEU261
A	MN301

---

site_id	AC4
Number of Residues	5
Details	binding site for residue MN B 301

Chain	Residue
B	GLU152
B	ASP185
B	HIS211
B	GLU246
B	PSJ303

---

site_id	AC5
Number of Residues	10
Details	binding site for residue EPE B 302

Chain	Residue
A	TRP264
A	ARG265
B	ARG128
B	ASN160
B	ILE161
B	ASN163
B	GLU167
B	HOH455
B	HOH539
E	LYS27

---

site_id	AC6
Number of Residues	12
Details	binding site for residue PSJ B 303

Chain	Residue
B	HIS12
B	SER69
B	VAL111
B	GLU152
B	GLU158
B	ASP185
B	HIS188
B	HIS211
B	ARG217
B	GLU246
B	LEU261
B	MN301

---

site_id	AC7
Number of Residues	5
Details	binding site for residue MN C 301

Chain	Residue
C	GLU152
C	ASP185
C	HIS211
C	GLU246
C	PSJ303

---

site_id	AC8
Number of Residues	10
Details	binding site for residue EPE C 302

Chain	Residue
B	LYS27
C	ARG128
C	ASN160
C	ILE161
C	ASN163
C	GLU167
C	HOH406
C	HOH525
D	TRP264
D	ARG265

---

site_id	AC9
Number of Residues	12
Details	binding site for residue PSJ C 303

Chain	Residue
C	HIS12
C	ALA43
C	SER69
C	GLU152
C	GLU158
C	ASP185
C	HIS188
C	HIS211
C	ARG217
C	GLU246
C	LEU261
C	MN301

---

site_id	AD1
Number of Residues	5
Details	binding site for residue MN D 301

Chain	Residue
D	GLU152
D	ASP185
D	HIS211
D	GLU246
D	PSJ303

---

site_id	AD2
Number of Residues	10
Details	binding site for residue EPE D 302

Chain	Residue
C	TRP264
C	ARG265
D	ARG128
D	ASN160
D	ILE161
D	ASN163
D	GLU167
D	HOH402
F	LYS27
F	HOH532

---

site_id	AD3
Number of Residues	15
Details	binding site for residue PSJ D 303

Chain	Residue
D	GLY110
D	VAL111
D	GLU152
D	GLU158
D	ASP185
D	HIS188
D	HIS211
D	ARG217
D	GLU246
D	LEU261
D	MN301
D	HIS12
D	ALA43
D	SER69
D	LEU70

---

site_id	AD4
Number of Residues	5
Details	binding site for residue MN E 301

Chain	Residue
E	GLU152
E	ASP185
E	HIS211
E	GLU246
E	PSJ303

---

site_id	AD5
Number of Residues	8
Details	binding site for residue EPE E 302

Chain	Residue
C	LYS27
E	ARG125
E	ARG128
E	ILE161
E	ASN163
E	GLU167
F	TRP264
F	ARG265

---

site_id	AD6
Number of Residues	12
Details	binding site for residue PSJ E 303

Chain	Residue
E	HIS12
E	SER69
E	LEU116
E	GLU152
E	GLU158
E	ASP185
E	HIS188
E	HIS211
E	ARG217
E	GLU246
E	LEU261
E	MN301

---

site_id	AD7
Number of Residues	5
Details	binding site for residue MN F 301

Chain	Residue
F	GLU152
F	ASP185
F	HIS211
F	GLU246
F	PSJ303

---

site_id	AD8
Number of Residues	10
Details	binding site for residue EPE F 302

Chain	Residue
A	LYS27
A	HOH562
E	TRP264
E	ARG265
F	ARG128
F	ILE161
F	ASN163
F	GLU167
F	HOH521
F	HOH557

---

site_id	AD9
Number of Residues	11
Details	binding site for residue PSJ F 303

Chain	Residue
F	HIS12
F	SER69
F	VAL111
F	GLU152
F	GLU158
F	ASP185
F	HIS188
F	HIS211
F	ARG217
F	GLU246
F	MN301

---

Functional Information from PROSITE/UniProt

site_id	PS00105
Number of Residues	14
Details	AA_TRANSFER_CLASS_1 Aminotransferases class-I pyridoxal-phosphate attachment site. GMAKsvLAaGDRLG

Chain	Residue	Details
A	GLY195-GLY208

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Proton donor/acceptor => ECO:0000250|UniProtKB:Q9WYP7

Chain	Residue	Details
A	GLU152
E	GLU246
F	GLU152
F	GLU246
A	GLU246
B	GLU152
B	GLU246
C	GLU152
C	GLU246
D	GLU152
D	GLU246
E	GLU152

---

site_id	SWS_FT_FI2
Number of Residues	30
Details	BINDING: BINDING => ECO:0000269|PubMed:33838083, ECO:0007744|PDB:7CJ5, ECO:0007744|PDB:7CJ9

Chain	Residue	Details
A	HIS12
B	ARG217
C	HIS12
C	SER69
C	GLU158
C	HIS188
C	ARG217
D	HIS12
D	SER69
D	GLU158
D	HIS188
A	SER69
D	ARG217
E	HIS12
E	SER69
E	GLU158
E	HIS188
E	ARG217
F	HIS12
F	SER69
F	GLU158
F	HIS188
A	GLU158
F	ARG217
A	HIS188
A	ARG217
B	HIS12
B	SER69
B	GLU158
B	HIS188

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269|PubMed:33838083, ECO:0000312|PDB:7CJ5, ECO:0007744|PDB:7CJ4, ECO:0007744|PDB:7CJ6, ECO:0007744|PDB:7CJ7, ECO:0007744|PDB:7CJ8, ECO:0007744|PDB:7CJ9

Chain	Residue	Details
A	GLU152
C	ASP185
C	HIS211
C	GLU246
D	GLU152
D	ASP185
D	HIS211
D	GLU246
E	GLU152
E	ASP185
E	HIS211
A	ASP185
E	GLU246
F	GLU152
F	ASP185
F	HIS211
F	GLU246
A	HIS211
A	GLU246
B	GLU152
B	ASP185
B	HIS211
B	GLU246
C	GLU152

---