7CGF

Crystal Structure of PUF-8 in Complex with PBE-RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	PUM-HD domain-containing protein	polymer	360	41905.1	1	UniProt (Q09487) Pfam (PF00806) In PDB	Caenorhabditis elegans
2	B	PBE-5G (5'-R(P*UP*GP*UP*AP*GP*AP*UP*A)-3')	polymer	8	2551.6	1		Caenorhabditis elegans
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4		water	water		18.0	204	Chemie (HOH)

Sequence modifications

A: 172 - 522 (UniProt: Q09487)

PDB	External Database	Details
Met 163	-	initiating methionine
Gly 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
Met 171	-	expression tag
Cys 318	Asn 318	engineered mutation
Arg 319	His 319	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	44456.6
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	44492.1

*Water molecules are not included.