7CGF
Crystal Structure of PUF-8 in Complex with PBE-RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.564, 89.564, 117.205 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.810 - 1.700 |
R-factor | 0.1996 |
Rwork | 0.198 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q0q |
RMSD bond length | 0.007 |
RMSD bond angle | 0.895 |
Data reduction software | XDS |
Data scaling software | HKL-2000 (V716.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
Rmerge | 0.088 | 0.068 | 0.562 |
Rmeas | 0.093 | 0.071 | 0.591 |
Rpim | 0.029 | 0.023 | 0.182 |
Total number of observations | 535738 | ||
Number of reflections | 53127 | 2927 | 2622 |
<I/σ(I)> | 4.8 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 10.1 | 9.4 | 10.2 |
CC(1/2) | 0.997 | 0.922 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 1M LiCl, 0.1M Citric acid (pH 5.0) and 15% PEG6000 |