7CFM
Cryo-EM structure of the P395-bound GPBAR-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39489.2 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 58 | 6375.3 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
| 4 | N | Nanobody-35 | polymer | 128 | 13885.4 | 1 | synthetic construct | ||
| 5 | R | G-protein coupled bile acid receptor 1 | polymer | 330 | 35275.2 | 1 | UniProt (Q8TDU6) Pfam (PF00001) In PDB | Homo sapiens (Human) | G-protein coupled receptor GPCR19,hGPCR19,Membrane-type receptor for bile acids,M-BAR,hBG37,BG37 |
| 6 | R | 2-(ethylamino)-6-[3-(4-propan-2-ylphenyl)propanoyl]-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-4-carboxamide | non-polymer | 395.5 | 1 | Chemie (FWX) | |||
| 7 | R | CHOLESTEROL | non-polymer | 386.7 | 3 | Chemie (CLR) | |||
| 8 | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 140708.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1555.5 | |
| All* | Total formula weight | 142264.0 |
