7C8O

Crystal structure of IscU D40A/H106A variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Nitrogen-fixing NifU domain protein	polymer	137	15153.4	4	UniProt (A0B757) Pfam (PF01592) In PDB	Methanothrix thermoacetophila	IscU
2	A, B	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
3		water	water		18.0	187	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 129 (UniProt: A0B757)

PDB	External Database	Details
Ala 40	Asp 40	engineered mutation
Ala 106	His 106	engineered mutation
Leu 130	-	expression tag
Glu 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	60613.5
Non-Polymers*	Number of molecules	2
	Total formula weight	351.6
All*	Total formula weight	60965.2

*Water molecules are not included.