7C8O
Crystal structure of IscU D40A/H106A variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.678, 60.376, 64.562 |
| Unit cell angles | 90.00, 95.78, 90.00 |
Refinement procedure
| Resolution | 42.260 - 1.840 |
| R-factor | 0.2008 |
| Rwork | 0.194 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z7e |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.135 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.260 | 1.860 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.091 | 0.910 |
| Rmeas | 0.099 | 0.983 |
| Rpim | 0.038 | 0.368 |
| Total number of observations | 271483 | |
| Number of reflections | 40163 | 3984 |
| <I/σ(I)> | 13.08 | 3.53 |
| Completeness [%] | 99.8 | 99.95 |
| Redundancy | 3.469 | 3.602 |
| CC(1/2) | 0.998 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 0.2 M Potassium iodide, 20 % (w/v) PEG 3350 |
