7C7L

Cryo-EM structure of the Cas12f1-sgRNA-target DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	CRISPR-associated protein Cas14a.1	polymer	539	62748.6	2	UniProt (A0A482D308) Pfam (PF07282) In PDB	uncultured archaeon	Cas12f.1
2	C	sgRNA	polymer	180	58133.5	1		uncultured archaeon
3	D	DNA (40-mer)	polymer	40	12298.0	1		synthetic construct
4	E	DNA (40-mer)	polymer	40	12323.9	1		synthetic construct
5	A, B	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

A, B: 1 - 529 (UniProt: A0A482D308)

PDB	External Database	Details
Met -9	-	initiating methionine
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 326	Asp 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	208252.6
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	208448.8

*Water molecules are not included.