7C7L
Cryo-EM structure of the Cas12f1-sgRNA-target DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | CRISPR-associated protein Cas14a.1 | polymer | 539 | 62748.6 | 2 | UniProt (A0A482D308) Pfam (PF07282) | uncultured archaeon | Cas12f.1 |
| 2 | C (C) | sgRNA | polymer | 180 | 58133.5 | 1 | uncultured archaeon | ||
| 3 | D (D) | DNA (40-mer) | polymer | 40 | 12298.0 | 1 | synthetic construct | ||
| 4 | E (E) | DNA (40-mer) | polymer | 40 | 12323.9 | 1 | synthetic construct | ||
| 5 | F, G, H (A, B) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 529 (UniProt: A0A482D308)
| PDB | External Database | Details |
|---|---|---|
| Met -9 | - | initiating methionine |
| Gly -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 326 | Asp 326 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 208252.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 208448.8 |
