7C7K

Crystal Structure of Thioacyl-Glyceraldehyde-3-phosphate dehydrogenase 1(GAPDH 1) from Escherichia coli at 1.77 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (O, P, Q, R)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	352	37887.9	4	UniProt (A0A140NCK4)	Escherichia coli BL21(DE3)
2	E, L, R, X (O, P, Q, R)	GLYCERALDEHYDE-3-PHOSPHATE	non-polymer		170.1	4	Chemie (G3H)
3	F, J (O)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	BA, G, H, I, M... (R, O, P, Q)	1,2-ETHANEDIOL	non-polymer		62.1	12	Chemie (EDO)
5	AA, K (R, O)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
6	CA, O, V (R, P, Q)	ACETONE	non-polymer		58.1	3	Chemie (ACN)
7	Q, Y (Q, R)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
8	DA, EA, FA, GA (O, P, Q, R)	water	water		18.0	1209	Chemie (HOH)

Sequence modifications

O, P, Q, R: 1 - 333 (UniProt: A0A140NCK4)

PDB	External Database	Details
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151551.7
Non-Polymers*	Number of molecules	25
	Total formula weight	3328.3
All*	Total formula weight	154880.0

*Water molecules are not included.