7C7K
Crystal Structure of Thioacyl-Glyceraldehyde-3-phosphate dehydrogenase 1(GAPDH 1) from Escherichia coli at 1.77 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9776 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.163, 90.163, 345.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.490 - 1.770 |
| R-factor | 0.14503 |
| Rwork | 0.143 |
| R-free | 0.18080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c5f |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.111 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.109 | 0.603 |
| Number of reflections | 139316 | 139316 |
| <I/σ(I)> | 35.9 | 4.75 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 26.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 100 mM sodium PBS pH 6.6, 16%(w/v) PEG 1000, 200mM MgCl2 |
