7C6W

Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritetraose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sugar ABC transporter, periplasmic sugar-binding protein	polymer	422	45906.2	1	UniProt (Q53W80) Pfam (PF13416) In PDB	Thermus thermophilus HB8
2	B	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		666.6	1	In PDB GlyTouCan (G75242TK)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	A	SULFUR DIOXIDE	non-polymer		64.1	1	Chemie (SO2)
5	A	CARBONATE ION	non-polymer		60.0	1	Chemie (CO3)
6	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
8		water	water		18.0	319	Chemie (HOH)

Sequence modifications

A: 1 - 415 (UniProt: Q53W80)

PDB	External Database	Details
Met 0	-	initiating methionine
Arg 174	Lys 192	engineered mutation
Thr 175	Asn 193	engineered mutation
Pro 176	Ser 194	engineered mutation
-	Trp 195	deletion
Arg 177	Asp 196	engineered mutation
Thr 178	Val 197	engineered mutation
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45906.2
Branched	Number of molecules	1
	Total formula weight	666.6
Non-Polymers*	Number of molecules	6
	Total formula weight	378.6
All*	Total formula weight	46951.4

*Water molecules are not included.