7C6W
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-06-29 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.110, 62.600, 109.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.510 - 1.880 |
R-factor | 0.1899 |
Rwork | 0.187 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c63 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.880 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 58.110 | 58.110 | 1.920 |
High resolution limit [Å] | 1.880 | 9.020 | 1.880 |
Rmerge | 0.081 | 0.028 | 0.463 |
Rmeas | 0.087 | 0.031 | 0.496 |
Rpim | 0.031 | 0.011 | 0.176 |
Total number of observations | 240945 | 2511 | 14248 |
Number of reflections | 32582 | 367 | 1978 |
<I/σ(I)> | 15.2 | 33.2 | 3.5 |
Completeness [%] | 98.5 | 99.9 | 95.6 |
Redundancy | 7.4 | 6.8 | 7.2 |
CC(1/2) | 0.998 | 0.999 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 0.2 M Ammonium sulphate, 40% PEG 8000 |