7C6W

Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-06-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	58.110, 62.600, 109.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.510 - 1.880
R-factor	0.1899
Rwork	0.187
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c63
RMSD bond length	0.013
RMSD bond angle	1.880
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.110	58.110	1.920
High resolution limit [Å]	1.880	9.020	1.880
Rmerge	0.081	0.028	0.463
Rmeas	0.087	0.031	0.496
Rpim	0.031	0.011	0.176
Total number of observations	240945	2511	14248
Number of reflections	32582	367	1978
<I/σ(I)>	15.2	33.2	3.5
Completeness [%]	98.5	99.9	95.6
Redundancy	7.4	6.8	7.2
CC(1/2)	0.998	0.999	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2 M Ammonium sulphate, 40% PEG 8000