7C5N

Crystal Structure of C150A+H177A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli at 2.0 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	O, P, Q, R	Glyceraldehyde-3-phosphate dehydrogenase	polymer	352	37788.8	4	UniProt (A0A140NCK4) Pfam (PF00044) Pfam (PF02800) In PDB	Escherichia coli BL21(DE3)
2	O, P, Q, R	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	O	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	O, P, Q, R	CHLORIDE ION	non-polymer		35.5	11	Chemie (CL)
5	P	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6		water	water		18.0	1112	Chemie (HOH)

Sequence modifications

O, P, Q, R: 1 - 333 (UniProt: A0A140NCK4)

PDB	External Database	Details
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 150	Cys 150	engineered mutation
Ala 177	His 177	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151155.2
Non-Polymers*	Number of molecules	17
	Total formula weight	3200.7
All*	Total formula weight	154355.9

*Water molecules are not included.