7C5N

Crystal Structure of C150A+H177A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli at 2.0 Angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000166	molecular_function	nucleotide binding
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005737	cellular_component	cytoplasm
O	0006006	biological_process	glucose metabolic process
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0042802	molecular_function	identical protein binding
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0000166	molecular_function	nucleotide binding
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005737	cellular_component	cytoplasm
P	0006006	biological_process	glucose metabolic process
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0042802	molecular_function	identical protein binding
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0000166	molecular_function	nucleotide binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005737	cellular_component	cytoplasm
Q	0006006	biological_process	glucose metabolic process
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0042802	molecular_function	identical protein binding
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0000166	molecular_function	nucleotide binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005737	cellular_component	cytoplasm
R	0006006	biological_process	glucose metabolic process
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0042802	molecular_function	identical protein binding
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	35
Details	binding site for residue NAD O 401

Chain	Residue
O	ASN7
O	LYS78
O	CYS96
O	THR97
O	GLY98
O	PHE99
O	SER120
O	ALA121
O	ALA150
O	ASN315
O	PHE319
O	GLY8
O	CL405
O	HOH507
O	HOH513
O	HOH564
O	HOH576
O	HOH581
O	HOH587
O	HOH601
O	HOH607
O	HOH609
O	PHE9
O	HOH612
O	HOH644
O	HOH661
O	HOH668
O	HOH669
O	HOH673
O	GLY10
O	ARG11
O	ILE12
O	ASN33
O	ASP34
O	LEU35

---

site_id	AC2
Number of Residues	8
Details	binding site for residue PO4 O 402

Chain	Residue
O	ALA201
O	HOH616
O	HOH636
P	ALA201
P	HOH645
Q	ALA201
R	ALA201
R	HOH603

---

site_id	AC3
Number of Residues	4
Details	binding site for residue CL O 403

Chain	Residue
O	LYS227
O	GLY228
O	HOH733
O	HOH797

---

site_id	AC4
Number of Residues	4
Details	binding site for residue CL O 404

Chain	Residue
O	ALA80
O	LYS81
O	ALA108
O	HOH771

---

site_id	AC5
Number of Residues	3
Details	binding site for residue CL O 405

Chain	Residue
O	THR180
O	NAD401
O	HOH646

---

site_id	AC6
Number of Residues	30
Details	binding site for residue NAD P 401

Chain	Residue
P	ASN7
P	GLY8
P	PHE9
P	GLY10
P	ARG11
P	ILE12
P	ASN33
P	ASP34
P	LYS78
P	CYS96
P	THR97
P	GLY98
P	SER120
P	ALA121
P	ALA150
P	THR180
P	ASN315
P	PHE319
P	CL404
P	HOH502
P	HOH523
P	HOH543
P	HOH597
P	HOH602
P	HOH610
P	HOH622
P	HOH629
P	HOH640
P	HOH655
P	HOH684

---

site_id	AC7
Number of Residues	2
Details	binding site for residue EDO P 402

Chain	Residue
P	GLU62
P	HOH522

---

site_id	AC8
Number of Residues	3
Details	binding site for residue CL P 403

Chain	Residue
P	LYS227
P	GLY228
P	HOH775

---

site_id	AC9
Number of Residues	3
Details	binding site for residue CL P 404

Chain	Residue
P	THR180
P	NAD401
P	HOH603

---

site_id	AD1
Number of Residues	4
Details	binding site for residue CL P 405

Chain	Residue
P	ALA80
P	LYS81
P	ALA108
P	HOH714

---

site_id	AD2
Number of Residues	33
Details	binding site for residue NAD Q 401

Chain	Residue
Q	ARG11
Q	ILE12
Q	ASN33
Q	ASP34
Q	LYS78
Q	CYS96
Q	THR97
Q	GLY98
Q	PHE99
Q	SER120
Q	ALA121
Q	ALA150
Q	THR180
Q	ASN315
Q	PHE319
Q	CL404
Q	HOH519
Q	HOH524
Q	HOH552
Q	HOH581
Q	HOH584
Q	HOH591
Q	HOH596
Q	HOH604
Q	HOH615
Q	HOH618
Q	HOH621
Q	HOH622
Q	HOH641
Q	HOH643
Q	ASN7
Q	GLY8
Q	GLY10

---

site_id	AD3
Number of Residues	2
Details	binding site for residue CL Q 402

Chain	Residue
Q	LYS227
Q	GLY228

---

site_id	AD4
Number of Residues	3
Details	binding site for residue CL Q 403

Chain	Residue
Q	ALA80
Q	LYS81
Q	HOH716

---

site_id	AD5
Number of Residues	3
Details	binding site for residue CL Q 404

Chain	Residue
Q	THR180
Q	NAD401
Q	HOH535

---

site_id	AD6
Number of Residues	33
Details	binding site for residue NAD R 401

Chain	Residue
R	ASN7
R	GLY8
R	PHE9
R	GLY10
R	ARG11
R	ILE12
R	ASN33
R	ASP34
R	LEU35
R	LYS78
R	CYS96
R	THR97
R	GLY98
R	PHE99
R	SER120
R	ALA121
R	ALA150
R	THR180
R	ASN315
R	PHE319
R	HOH513
R	HOH521
R	HOH530
R	HOH550
R	HOH567
R	HOH586
R	HOH587
R	HOH600
R	HOH604
R	HOH609
R	HOH625
R	HOH662
R	HOH666

---

site_id	AD7
Number of Residues	4
Details	binding site for residue CL R 402

Chain	Residue
Q	HOH571
R	LYS227
R	GLY228
R	HOH675

---

site_id	AD8
Number of Residues	3
Details	binding site for residue CL R 403

Chain	Residue
R	THR180
R	THR182
R	HOH550

---

Functional Information from PROSITE/UniProt

site_id	PS00430
Number of Residues	89
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. hhhhhhssglvprgshmasmskvgingfgrigrlvlrrllevksnidvvaindltspkilayllkhdsnygpfpwsvdfte..................................DSLIVDGK

Chain	Residue	Details
O	HIS-18-LYS70

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