7C5I

Crystal Structure of C150A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with PO4 at 2.49 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (O, P, Q, R)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	352	37855.9	4	UniProt (A0A140NCK4) Pfam (PF00044) Pfam (PF02800)	Escherichia coli BL21(DE3)
2	E, M, R, X (O, P, Q, R)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	AA, F, G, H, I... (R, O, P, Q)	PHOSPHATE ION	non-polymer		95.0	13	Chemie (PO4)
4	CA, J, K, Q, W (R, O, P, Q)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
5	BA, L, V (R, O, Q)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
6	DA, EA, FA, GA (O, P, Q, R)	water	water		18.0	557	Chemie (HOH)

Sequence modifications

O, P, Q, R: 1 - 333 (UniProt: A0A140NCK4)

PDB	External Database	Details
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 150	Cys 150	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151423.4
Non-Polymers*	Number of molecules	25
	Total formula weight	4251.8
All*	Total formula weight	155675.2

*Water molecules are not included.