7C5I

Crystal Structure of C150A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with PO4 at 2.49 Angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0006006	biological_process	glucose metabolic process
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0006006	biological_process	glucose metabolic process
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0006006	biological_process	glucose metabolic process
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0006006	biological_process	glucose metabolic process
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	31
Details	binding site for residue NAD O 401

Chain	Residue
O	ASN7
O	LYS78
O	CYS96
O	THR97
O	GLY98
O	PHE99
O	SER120
O	ALA121
O	THR180
O	ASN315
O	PHE319
O	GLY8
O	PO4403
O	PO4404
O	HOH510
O	HOH515
O	HOH528
O	HOH530
O	HOH534
O	HOH569
O	HOH582
O	HOH601
O	PHE9
O	HOH611
O	HOH613
O	GLY10
O	ARG11
O	ILE12
O	ASN33
O	ASP34
O	LEU35

---

site_id	AC2
Number of Residues	5
Details	binding site for residue PO4 O 402

Chain	Residue
O	HIS163
O	GLY167
O	ILE168
O	GLU169
O	LYS225

---

site_id	AC3
Number of Residues	6
Details	binding site for residue PO4 O 403

Chain	Residue
O	THR180
O	THR182
O	ARG232
O	NAD401
O	PO4404
O	HOH515

---

site_id	AC4
Number of Residues	6
Details	binding site for residue PO4 O 404

Chain	Residue
O	SER149
O	ALA150
O	THR151
O	HIS177
O	NAD401
O	PO4403

---

site_id	AC5
Number of Residues	8
Details	binding site for residue PO4 O 405

Chain	Residue
O	ALA201
O	HOH571
O	HOH572
P	ALA201
P	HOH524
Q	ALA201
Q	HOH585
R	ALA201

---

site_id	AC6
Number of Residues	2
Details	binding site for residue CL O 406

Chain	Residue
O	GLY228
O	HOH640

---

site_id	AC7
Number of Residues	3
Details	binding site for residue CL O 407

Chain	Residue
O	ALA80
O	LYS81
O	ALA108

---

site_id	AC8
Number of Residues	2
Details	binding site for residue EDO O 408

Chain	Residue
O	HOH655
R	PHE60

---

site_id	AC9
Number of Residues	29
Details	binding site for residue NAD P 401

Chain	Residue
P	ASN7
P	GLY8
P	PHE9
P	GLY10
P	ARG11
P	ILE12
P	ASN33
P	ASP34
P	LEU35
P	LYS78
P	CYS96
P	THR97
P	GLY98
P	PHE99
P	SER120
P	ALA121
P	ALA150
P	ASN315
P	PHE319
P	PO4403
P	PO4404
P	HOH501
P	HOH523
P	HOH525
P	HOH542
P	HOH543
P	HOH545
P	HOH579
P	HOH594

---

site_id	AD1
Number of Residues	5
Details	binding site for residue PO4 P 402

Chain	Residue
P	HIS163
P	GLY167
P	ILE168
P	LEU222
P	LYS225

---

site_id	AD2
Number of Residues	6
Details	binding site for residue PO4 P 403

Chain	Residue
P	ARG232
P	NAD401
P	PO4404
P	HOH543
P	THR180
P	THR182

---

site_id	AD3
Number of Residues	7
Details	binding site for residue PO4 P 404

Chain	Residue
P	SER149
P	ALA150
P	THR151
P	HIS177
P	NAD401
P	PO4403
P	HOH567

---

site_id	AD4
Number of Residues	4
Details	binding site for residue CL P 405

Chain	Residue
O	HOH532
P	LYS227
P	GLY228
P	HOH547

---

site_id	AD5
Number of Residues	32
Details	binding site for residue NAD Q 401

Chain	Residue
Q	ASN7
Q	GLY8
Q	PHE9
Q	GLY10
Q	ARG11
Q	ILE12
Q	ASN33
Q	ASP34
Q	LYS78
Q	CYS96
Q	THR97
Q	GLY98
Q	PHE99
Q	SER120
Q	ALA121
Q	ALA150
Q	THR180
Q	ASN315
Q	PHE319
Q	PO4403
Q	PO4404
Q	HOH501
Q	HOH518
Q	HOH519
Q	HOH525
Q	HOH528
Q	HOH534
Q	HOH542
Q	HOH553
Q	HOH562
Q	HOH591
Q	HOH604

---

site_id	AD6
Number of Residues	5
Details	binding site for residue PO4 Q 402

Chain	Residue
Q	HIS163
Q	GLY167
Q	ILE168
Q	GLU221
Q	LYS225

---

site_id	AD7
Number of Residues	6
Details	binding site for residue PO4 Q 403

Chain	Residue
Q	THR180
Q	THR182
Q	ARG232
Q	NAD401
Q	PO4404
Q	HOH562

---

site_id	AD8
Number of Residues	6
Details	binding site for residue PO4 Q 404

Chain	Residue
Q	SER149
Q	ALA150
Q	THR151
Q	HIS177
Q	NAD401
Q	PO4403

---

site_id	AD9
Number of Residues	3
Details	binding site for residue EDO Q 405

Chain	Residue
Q	ARG19
Q	HIS286
Q	ARG325

---

site_id	AE1
Number of Residues	4
Details	binding site for residue CL Q 406

Chain	Residue
Q	LYS227
Q	GLY228
Q	HOH612
R	HOH512

---

site_id	AE2
Number of Residues	30
Details	binding site for residue NAD R 401

Chain	Residue
R	ASN7
R	GLY8
R	PHE9
R	GLY10
R	ARG11
R	ILE12
R	ASN33
R	ASP34
R	LEU35
R	LYS78
R	CYS96
R	GLY98
R	PHE99
R	SER120
R	ALA121
R	ALA150
R	ASN315
R	PHE319
R	PO4403
R	PO4404
R	HOH502
R	HOH508
R	HOH514
R	HOH527
R	HOH537
R	HOH539
R	HOH548
R	HOH554
R	HOH573
R	HOH581

---

site_id	AE3
Number of Residues	5
Details	binding site for residue PO4 R 402

Chain	Residue
R	HIS163
R	GLY167
R	ILE168
R	GLU221
R	LYS225

---

site_id	AE4
Number of Residues	5
Details	binding site for residue PO4 R 403

Chain	Residue
R	THR180
R	THR182
R	ARG232
R	NAD401
R	PO4404

---

site_id	AE5
Number of Residues	6
Details	binding site for residue PO4 R 404

Chain	Residue
R	SER149
R	ALA150
R	THR151
R	HIS177
R	NAD401
R	PO4403

---

site_id	AE6
Number of Residues	3
Details	binding site for residue EDO R 405

Chain	Residue
R	ARG19
R	HIS286
R	ARG325

---

site_id	AE7
Number of Residues	3
Details	binding site for residue CL R 406

Chain	Residue
Q	HOH592
R	GLY228
R	HOH572

---

Functional Information from PROSITE/UniProt

site_id	PS00430
Number of Residues	89
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. hhhhhhssglvprgshmasmskvgingfgrigrlvlrrllevksnidvvaindltspkilayllkhdsnygpfpwsvdfte..................................DSLIVDGK

Chain	Residue	Details
O	HIS-18-LYS70

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