7C5H

Crystal Structure of Glyceraldehyde-3-phosphate dehydrogenase1 from Escherichia coli at 2.09 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (O, P, Q, R)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	352	37887.9	4	UniProt (A0A140NCK4) Pfam (PF00044) Pfam (PF02800)	Escherichia coli BL21(DE3)
2	BA, CA, E, G, M... (R, O, P, Q)	PHOSPHATE ION	non-polymer		95.0	9	Chemie (PO4)
3	AA, F, N, U (R, O, P, Q)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
4	EA, FA, H, K, L... (R, O, P, Q)	CHLORIDE ION	non-polymer		35.5	7	Chemie (CL)
5	DA, I, J, Q, R... (R, O, P, Q)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
6	P (P)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	W (Q)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8	GA, HA, IA, JA (O, P, Q, R)	water	water		18.0	881	Chemie (HOH)

Sequence modifications

O, P, Q, R: 1 - 333 (UniProt: A0A140NCK4)

PDB	External Database	Details
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151551.7
Non-Polymers*	Number of molecules	28
	Total formula weight	4327.2
All*	Total formula weight	155878.9

*Water molecules are not included.