7C5H
Crystal Structure of Glyceraldehyde-3-phosphate dehydrogenase1 from Escherichia coli at 2.09 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.778, 89.778, 340.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.090 |
| R-factor | 0.15336 |
| Rwork | 0.151 |
| R-free | 0.20274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c5f |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.937 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.154 | 0.604 |
| Number of reflections | 83301 | 83301 |
| <I/σ(I)> | 13.28 | 3.14 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 13 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 100mM sodium PBS pH 6.1, 16%(w/v) PEG 1000, 200mM MgCl2 |
