7C5G
Crystal Structure of C150S mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with PO4 at 1.98 Angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (O, P, Q, R) | Glyceraldehyde-3-phosphate dehydrogenase | polymer | 352 | 37871.9 | 4 | UniProt (A0A140NCK4) Pfam (PF00044) Pfam (PF02800) | Escherichia coli BL21(DE3) | |
| 2 | E, I, M, Q (O, P, Q, R) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 4 | Chemie (NAD) | |||
| 3 | F, J, N, R, S (O, P, Q, R) | PHOSPHATE ION | non-polymer | 95.0 | 5 | Chemie (PO4) | |||
| 4 | G, H, K, L, P... (O, P, Q, R) | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 5 | O (Q) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | U, V, W, X (O, P, Q, R) | water | water | 18.0 | 1021 | Chemie (HOH) |
Sequence modifications
O, P, Q, R: 1 - 333 (UniProt: A0A140NCK4)
| PDB | External Database | Details |
|---|---|---|
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Pro -7 | - | expression tag |
| Arg -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Ser -4 | - | expression tag |
| His -3 | - | expression tag |
| Met -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ser 150 | Cys 150 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 151487.4 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 3403.3 | |
| All* | Total formula weight | 154890.8 |
