7C5G

Crystal Structure of C150S mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli complexed with PO4 at 1.98 Angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000166	molecular_function	nucleotide binding
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005737	cellular_component	cytoplasm
O	0006006	biological_process	glucose metabolic process
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0042802	molecular_function	identical protein binding
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0000166	molecular_function	nucleotide binding
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005737	cellular_component	cytoplasm
P	0006006	biological_process	glucose metabolic process
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0042802	molecular_function	identical protein binding
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding
Q	0000166	molecular_function	nucleotide binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005737	cellular_component	cytoplasm
Q	0006006	biological_process	glucose metabolic process
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0042802	molecular_function	identical protein binding
Q	0050661	molecular_function	NADP binding
Q	0051287	molecular_function	NAD binding
R	0000166	molecular_function	nucleotide binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005737	cellular_component	cytoplasm
R	0006006	biological_process	glucose metabolic process
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0042802	molecular_function	identical protein binding
R	0050661	molecular_function	NADP binding
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	35
Details	binding site for residue NAD O 401

Chain	Residue
O	ASN7
O	LYS78
O	CYS96
O	THR97
O	GLY98
O	PHE99
O	SER120
O	ALA121
O	SER150
O	ASN315
O	PHE319
O	GLY8
O	PO4402
O	HOH522
O	HOH543
O	HOH549
O	HOH561
O	HOH562
O	HOH566
O	HOH601
O	HOH618
O	HOH634
O	PHE9
O	HOH647
O	HOH654
O	HOH688
O	HOH696
O	HOH697
O	HOH702
O	GLY10
O	ARG11
O	ILE12
O	ASN33
O	ASP34
O	LEU35

---

site_id	AC2
Number of Residues	8
Details	binding site for residue PO4 O 402

Chain	Residue
O	THR180
O	THR182
O	ARG232
O	NAD401
O	HOH549
O	HOH566
O	HOH702
O	HOH726

---

site_id	AC3
Number of Residues	4
Details	binding site for residue CL O 403

Chain	Residue
O	LYS227
O	GLY228
O	HOH728
O	HOH778

---

site_id	AC4
Number of Residues	5
Details	binding site for residue CL O 404

Chain	Residue
O	HIS177
O	HIS207
O	ALA230
O	ARG232
O	HOH646

---

site_id	AC5
Number of Residues	32
Details	binding site for residue NAD P 401

Chain	Residue
P	ASN7
P	GLY8
P	GLY10
P	ARG11
P	ILE12
P	ASN33
P	ASP34
P	LEU35
P	LYS78
P	CYS96
P	THR97
P	GLY98
P	PHE99
P	SER120
P	ALA121
P	SER150
P	ASN315
P	PHE319
P	PO4402
P	HOH502
P	HOH505
P	HOH507
P	HOH515
P	HOH554
P	HOH588
P	HOH604
P	HOH614
P	HOH636
P	HOH656
P	HOH657
P	HOH663
P	HOH686

---

site_id	AC6
Number of Residues	6
Details	binding site for residue PO4 P 402

Chain	Residue
P	THR180
P	THR182
P	ARG232
P	NAD401
P	HOH507
P	HOH686

---

site_id	AC7
Number of Residues	3
Details	binding site for residue CL P 403

Chain	Residue
P	LYS227
P	GLY228
P	HOH717

---

site_id	AC8
Number of Residues	5
Details	binding site for residue CL P 404

Chain	Residue
P	HIS177
P	HIS207
P	ALA230
P	ARG232
P	HOH615

---

site_id	AC9
Number of Residues	34
Details	binding site for residue NAD Q 401

Chain	Residue
Q	GLY8
Q	PHE9
Q	GLY10
Q	ARG11
Q	ILE12
Q	ASN33
Q	ASP34
Q	LYS78
Q	CYS96
Q	THR97
Q	GLY98
Q	PHE99
Q	SER120
Q	ALA121
Q	ASN315
Q	PHE319
Q	PO4402
Q	HOH501
Q	HOH514
Q	HOH517
Q	HOH518
Q	HOH531
Q	HOH536
Q	HOH570
Q	HOH573
Q	HOH595
Q	HOH597
Q	HOH612
Q	HOH618
Q	HOH633
Q	HOH667
Q	HOH681
Q	HOH688
Q	ASN7

---

site_id	AD1
Number of Residues	7
Details	binding site for residue PO4 Q 402

Chain	Residue
Q	THR180
Q	THR182
Q	ARG232
Q	NAD401
Q	HOH517
Q	HOH595
Q	HOH667

---

site_id	AD2
Number of Residues	4
Details	binding site for residue EDO Q 403

Chain	Residue
Q	SER25
Q	ASN26
Q	ILE27
Q	ASP28

---

site_id	AD3
Number of Residues	3
Details	binding site for residue CL Q 404

Chain	Residue
Q	LYS227
Q	GLY228
Q	HOH708

---

site_id	AD4
Number of Residues	33
Details	binding site for residue NAD R 401

Chain	Residue
R	ASN7
R	GLY8
R	PHE9
R	GLY10
R	ARG11
R	ILE12
R	ASN33
R	ASP34
R	LYS78
R	CYS96
R	THR97
R	GLY98
R	PHE99
R	SER120
R	ALA121
R	ASN315
R	PHE319
R	PO4402
R	HOH505
R	HOH508
R	HOH511
R	HOH524
R	HOH526
R	HOH528
R	HOH530
R	HOH551
R	HOH556
R	HOH568
R	HOH576
R	HOH620
R	HOH631
R	HOH634
R	HOH644

---

site_id	AD5
Number of Residues	9
Details	binding site for residue PO4 R 402

Chain	Residue
R	THR180
R	THR182
R	ARG232
R	NAD401
R	PO4403
R	HOH502
R	HOH505
R	HOH528
R	HOH634

---

site_id	AD6
Number of Residues	9
Details	binding site for residue PO4 R 403

Chain	Residue
R	SER149
R	SER150
R	THR151
R	HIS177
R	THR209
R	ARG232
R	PO4402
R	HOH501
R	HOH528

---

site_id	AD7
Number of Residues	3
Details	binding site for residue CL R 404

Chain	Residue
Q	HOH579
R	LYS227
R	GLY228

---

Functional Information from PROSITE/UniProt

site_id	PS00430
Number of Residues	89
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. hhhhhhssglvprgshmasmskvgingfgrigrlvlrrllevksnidvvaindltspkilayllkhdsnygpfpwsvdfte..................................DSLIVDGK

Chain	Residue	Details
O	HIS-18-LYS70

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