7C3E

Crystal structure of R97A/R150A/R203A mutant of AofleA from Arthrobotrys oligospora

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	AofleA	polymer	355	39213.9	4	UniProt (G1XA82) Pfam (PF07938)	Arthrobotrys oligospora (strain ATCC 24927 / CBS 115.81 / DSM 1491) (Nematode-trapping fungus)
2	AA, BA, CA, DA, E... (C, A, D, B)	GLYCEROL	non-polymer		92.1	24	Chemie (GOL)
3	GA, HA, L, M, N... (C, A, D, B)	SULFATE ION	non-polymer		96.1	15	Chemie (SO4)
4	RA, SA, TA, UA (A, B, C, D)	water	water		18.0	1500	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 343 (UniProt: G1XA82)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag
Ala 97	Arg 97	engineered mutation
Ala 150	Arg 150	engineered mutation
Ala 203	Arg 203	engineered mutation
Ala 344	-	expression tag
Ala 345	-	expression tag
Ala 346	-	expression tag
Leu 347	-	expression tag
Glu 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	156855.6
Non-Polymers*	Number of molecules	39
	Total formula weight	3651.2
All*	Total formula weight	160506.8

*Water molecules are not included.