7C2I

Crystal structure of nsp16-nsp10 heterodimer from SARS-CoV-2 in complex with SAM (with additional SAM during crystallization)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	2'-O-methyltransferase	polymer	305	34315.3	1	UniProt (P0DTD1) Pfam (PF06460) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,Non-structural protein 16,nsp16,Replicase polyprotein 1ab
2	B	Non-structural protein 10	polymer	144	15214.4	1	UniProt (P0DTD1) Pfam (PF09401) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	pp1ab,ORF1ab polyprotein,nsp10,Growth factor-like peptide,GFL,Replicase polyprotein 1ab
3	A	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
4	B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5		water	water		18.0	164	Chemie (HOH)

Sequence modifications

A: 1 - 298 (UniProt: P0DTD1)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

B: 1 - 139 (UniProt: P0DTD1)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49529.7
Non-Polymers*	Number of molecules	3
	Total formula weight	529.3
All*	Total formula weight	50059.0

*Water molecules are not included.