7C27
Glycosidase F290Y at pH4.5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isomaltose glucohydrolase | polymer | 405 | 44060.1 | 1 | UniProt (D2PPM8) Pfam (PF00723) | Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399) | |
| 2 | B, C, D, E (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 3 | F (A) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) | |||
| 4 | G (A) | AMMONIUM ION | non-polymer | 18.0 | 1 | Chemie (NH4) | |||
| 5 | H (A) | water | water | 18.0 | 276 | Chemie (HOH) |
Sequence modifications
A: 1 - 385 (UniProt: D2PPM8)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Tyr 290 | Phe 290 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 44060.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 578.5 | |
| All* | Total formula weight | 44638.6 |
