7C27
Glycosidase F290Y at pH4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-06 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 104.811, 104.811, 90.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.920 - 1.910 |
| Rwork | 0.182 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z3d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.623 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.920 | 2.020 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmeas | 0.242 | 3.722 |
| Number of reflections | 39724 | 6298 |
| <I/σ(I)> | 0.107 | 0.01 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 26.4 | 26.7 |
| CC(1/2) | 0.998 | 0.429 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M Ammonium citrate (pH 7.0), 8% PEG monomethyl ether 2000 |
