7BZ2

Cryo-EM structure of the formoterol-bound beta2 adrenergic receptor-Gs protein complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	395	45812.6	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37346.8	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (Bovine)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	78	8805.2	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
4	D (N)	Nb35 nanobody	polymer	161	17483.7	1		Lama glama
5	E (R)	Beta2 adrenergic receptor	polymer	367	42010.8	1	Pfam (PF00001) UniProt (by SIFTS) (P07550)	Homo sapiens
6	F (R)	~{N}-[5-[(1~{R})-2-[[(2~{R})-1-(4-methoxyphenyl)propan-2-yl]amino]-1-oxidanyl-ethyl]-2-oxidanyl-phenyl]methanamide	non-polymer		344.4	1	Chemie (H98)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Ser 0	-	expression tag

B: 1 - 340 (UniProt: P62871)

PDB	External Database	Details
Ser 304	Arg 304	conflict

G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 68	Cys 68	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	151459.1
Non-Polymers*	Number of molecules	1
	Total formula weight	344.4
All*	Total formula weight	151803.5

*Water molecules are not included.