7BY0

The cryo-EM structure of CENP-A nucleosome in complex with the phosphorylated CENP-C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.2,Histone H3-like centromeric protein A	polymer	141	16089.9	2	UniProt (Q6XXM1) Pfam (PF00125) UniProt (by SIFTS) (P84229) In PDB	Gallus gallus (Chicken)	Centromere protein A,GgCENP-A
2	B, F	Histone H4	polymer	102	11337.4	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (Human)
3	C, G	Histone H2A type 1-B/E	polymer	130	14165.6	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H	Histone H2B type 1-J	polymer	126	13935.2	2	UniProt (P06899) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I	DNA (145-MER)	polymer	145	44520.4	1		synthetic construct
6	J	DNA (145-MER)	polymer	145	44991.7	1		synthetic construct
7	K, L	Maltose Binding Protein tag, linker,CENP-C	polymer	664	73796.3	2	UniProt (O57393) Pfam (PF01547) Pfam (PF11699) UniProt (by SIFTS) (P0AEX9) In PDB	unidentified cloning vector

Sequence modifications

A, E: 1 - 64 (PDB: 7BY0)
A, E: 65 - 141 (UniProt: Q6XXM1)
K, L: 201 - 611 (PDB: 7BY0)
K, L: 612 - 864 (UniProt: O57393)

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	348160.9
All*	Total formula weight	348160.9

*Water molecules are not included.