7BY0
The cryo-EM structure of CENP-A nucleosome in complex with the phosphorylated CENP-C
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | JEOL CRYO ARM 200 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 1.4 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.5 |
Number of Particle | 343497 |
Software | RELION,Gctf |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |